The FBI has requested key evidence, including a glove and DNA found in Nancy Guthrie’s Tucson home, to process at its national crime lab in Quantico, Virginia, but Pima County Sheriff Chris Nanos is insisting the items be sent to a private lab in Florida instead.

Federal officials say this decision risks delaying the investigation and criticized the sheriff’s office for not involving the FBI sooner.

The Pima County Sheriff’s Office remains the lead agency in the case, limiting the FBI’s role to requests for assistance.

Surveillance footage released earlier this week shows a masked suspect approaching Guthrie’s front door before her disappearance on February 1, 2026, Fox News has reported.

The FBI has increased the reward to $100,000 for information leading to her return or an arrest, while tensions between the federal and local authorities grow amid the ongoing investigation.